ISOLATION AND FERMENTATION CONDITIONS OF SOIL FUNGUS PP-15 FROM GAWECHAUNG FORT
Abstract
- Soil samples were collected from six different places of Gawechaung fort, Magway Township, Magway Region. Thirty fungi were isolated from these six different soil samples. Isolations of fungi were undertaken by the serial dilution method and cultured by using Blakeslee’s Malt Extract Agar (BMEA Medium), Czapek-Dox Agar (CZA Medium) and Malt Extract Agar (MEA Medium). Isolated fungi were given as PP-1 to PP-30. Antimicrobial activities of these fungi were evaluated by agar well diffusion assay with seven test organisms. Among them, ten fungal strains showed the antimicrobial activity.Especially, PP-15 gave the best antibacterial activity on Agrobacterium tumefaciens NTTE 09678.Therefore, different fermentation parameters of PP-15 were studied by the fermentation period, proper age and size of inoculums, effect of various carbon and nitrogen sources, pH, temperature, fermentation medium, shaker and static on Agrobacterium tumefacines.
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Year
- 2020
Author
-
Phyu Phyu Aung
Subject
- Botany and Marine
Publisher
- Myanmar Academy of Arts and Science (MAAS)