ISOLATION AND FERMENTATION CONDITIONS OF SELECTED SOIL FUNGUS TT-27 BETWEEN YENANCHAUNG AND MAGWAY TOWNSHIP, MAGWAY REGION
Abstract
- Six soil samples were collected from six different places of Magway and Yenanchaung Township, Magway Region. Twenty-nine fungi were isolated from these soil samples. Isolation of soil fungi were carried out in Biotechnology Development Center (BDC) from Pathein University. Isolations of fungi were undertaken by the serial dilution method, and cultured on Blakeslee’s Malt Extract Agar (BMEA Medium), Czapek-Dox Agar (CZA Medium) and Malt Extract Agar (MEA Medium). The isolated fungi were given as TT-1 to TT-29. In the preliminary study of antimicrobial activity, all fungi were evaluated by agar well diffusion method with seven test organisms. Among them, four fungi TT-22, 23, 24 and 27 exhibited the highest antimicrobial activities. Especially, TT-27 showed the highest antimicrobial activity (39.10mm) on Escherichia coli. Therefore TT-27 was selected and the fermentation conditions of TT-27 were carried out by the study of fermentation period, proper age, size, different carbon and nitrogen sources, fermentation medium (FM), effects of pH, temperature, static and shaking culture.
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Year
- 2020
Author
-
Thida Than
Subject
- Botany and Marine
Publisher
- Myanmar Academy of Arts and Science (MAAS)